This paper describes the Shockley-Quiesser Limit (fundamental concepts that define solar cell efficiency) and is written by none other than Shockley and Quiesser. It's a groundbreaking work (good ol' 1960s) and I would love love love to get its full text.
From the pages of my lab notebook: Friday, December 20
In each well of the Western blot gel, the max amount that can be put in is 40 ul. Since I have 7 samples, and I will be doing a duplicate, I will make up 60ul lysate + 30ul dye for each sample.
Just like DNA gels, a ladder/protein marker is used as a standard to compare protein sizes in kDA (kiilodalton). Pour SDS buffer in the gel box (SDS running buffer equalizes the negative charge on the proteins). Put protein samples on a heat block for 5-10 minutes to denature the protein.
From the pages of my lab notebook: Thursday, December 19, 2013
Purpose of western blot: to check for transgene expression; expression of the NTRK1 or NTRK2 gene in the BaF3 cells (side note: I have an answer to the why model human leukemia in mouse question, to be found in my research paper)
Last week: lysed the cells with cell lysis buffer
Goal for today: to do a bovine serum albumin (BSA) protein assay--with BCA (bicinchoninic acid), a chemical reagent that colors the protein
'Twas the week before Christmas...The lab was holiday-ified with lights hanging across lab benches and sticker stars covering the windows. A cardboard box nativity scene rested on the work bench near the printer (think: sheep=T25 flasks with PCR tubes as legs).
After finishing my preparations for the western blot on Thursday, Dec. 19, my mentor told me to follow her to the tissue culture room--to see the cells from the IL3-withdrawal assay that I believed to have failed.
I re-read the NWSE/ISEF guidelines and I am wondering if I should be filling out continuation paperwork. If so, I could do that January 6-7th.
The thing is, I am not using any information from last year's project (new material PbS instead of last year's PbSe, totally new algorithms and model, multijunction aspect completely revises my procedure), so this is not a continuation project.
But will the judges think it should be a continuation? In other words, will I be disqualified if somebody thinks this should be a continuation?