Take a look at the attached file: this is an example of a DNA sequence trace file.
In this pdf, we see part of a DNA sequence of a mutant plasmid (about 1180 nucleotides). The purpose of this trace file is to check if the insert (mutation) was transferred into the plasmid or not.
To verify this, we just need to know that there is a nice, tall peak at the spot where the mutation is supposed to be. Then, based on the color of the peak, we can also see if the correct nucleotide (A, T, C, or G) was replaced.
(black=G, red=T, blue=C, green=A)
Ruland, Andres, Schulz-Drost, Christian, Sgobba, Vito, and Guldi, Dirk M. (2011). "Enhancing Photocurrent Efficiencies by Resonance Energy Transfer in CdTe Quantum Dot Multilayers: Towards Rainbow Solar Cells" in Advanced Materials.
Motivation of the paper:
- want a "low-cost, easily producible, and efficient" solar cell
- because current designs are not yet commercially viable, these researchers are trying to find a solution
- they decided to jump on the quantum dot (nanocrystal) bandwagon
Over the weekend, I read over the long packet I got from Marion. Most of it was specific sampling procedures for different types of things that might be in streams. Chemical components, biotic factors, and physical factors will be the three things I'll be focusing on.
The basic chemical measurements that I want to take in the field are the following:
dissolved O2 --> biochemical O2 demand?
Tomorrow, I need to figure out what we have in terms of equipment for measuring these.
Cells are like pets.
They need to be taken care of, and they need to be replenished and fed. Cells make friends when they are in a compact and limited space with other cells (ex. a 10cm diameter dish), and it's nice when there aren't a lot of other cells around, but if it gets too populated, the cells get tired of it (they get annoyed, pile up on each other, and fight for food!).