So I don't have my notes on hand as I left them at school, but stay with me, I remember the general points.
HA is produced in the cell membrane and secreted out into the ECM. The exact mechanisms by which HA interacts with MMPs are unknown at this time.
4MU functions by depleting the resource of one of the sugar building blocks of HA, as well as blocking the mRNA path that leads to the production of HA.
Plans for tomorrow: class first period, will turn in all forms and measure out leaves for new packs.
I was intending to put together the packs today, but forgot that I had a meeting about my outdoor trip. I'll take the packs tomorrow after school and put them in the stream, and bring the buckets with the old packs on Friday morning.
I won't be in class Friday, my trip leaves at 8:00 am.
Submitted by Nick Petty on Fri, 01/17/2014 - 10:32am
So I've added the chart showing the protein concentration found in my samples along with the respective amount of each sample I needed to add to each well when running the Western Blot. I'd love to get some more pictures incorporated into my presentation, and suggestions towards that would be awesome! I have sent an email to Kate about looking at the RT-PCR results with her, and then I can incorporate that into my results!