Yesterday, I started on my next steps to further investigate the unexpected behavior of the NTRK1 wild type and continue my research.
First, I prepared the plasmid sample and sequencing primers to do a full DNA sequence of the NTRK1 WT construct. The sequence should come back tomorrow.
Also, yesterday I had the opportunity to listen to a talk given by Dr. Kimberly Stegmaier from the Dana-Farber Cancer Institute. Her talk was great and she described her lab's work on studying small molecule targets for cancer therapy as well as their work in looking at cell differentiation and transcription factors--the small things and details that happen in the nucleus.
She shared findings from her lab about acute myeloid leukemia (AML), acute lymphoblastic leukemia (ALL), and neuroblastoma. In the AML and ALL research, the lab primarily focuses on pediatric patients. By profession, she is a pediatric oncologist, and that makes her work even more special.
In addition, their lab looks at known hits and tries to find out more about them. For example, in neuroblastoma, a particular gene called MYCN has been well known to be overexpressed and amplified in neuroblastoma tumors. Instead of looking for other targets, they examined closely how MYCN interacts with other proteins.
They also use a mouse model in their lab, and study different chemical compounds that may inhibit certain gene expression or activity of oncogenes. Additionally, her research has passed on to significant clinical trials.
Many survival rates in both children and adults can be better, and that is what makes the research I am doing, the research Dr. Stegmaier is doing, and the research other labs are doing so meaningful.
I was inspired by her great depth of knowledge across all cancers and thoughtful answers to the questions asked at the end of her presentation.
Debriefing Gresham-Barlow. Preparing for the Intel NWSE & ISEF.
- Poster design was inefficient. Next time, I have resolved to just use rulers and tape instead of the fancy metal screws I bought from Home Depot. (Read that on the internet) I'm not as worried about efficiency in poster setup for the state fair because I will go the night before to setup, but the screws also forced me to bore the holes, which were imprecise. Thus, rulers and tape, rulers and tape, all the way.
- The judges checked off everything on the comment sheet, so at this point I am just looking to fine-tune my oral presentation. No concerns about the content of my project. The written criticism was to eliminate the bibliography from my poster. I already printed my poster, so I'm caught between redesigning to address this concern and paying another $100 to print my poster, or just leaving my references on there at the state fair and ISEF. What do you think?
- I need to revise my research paper. As I was talking to the judges, I noticed that I could improve my JAVA program by incorporating an automated design process, e.g. instead of identifying each solar panel design manually (as I did before), I am now thinking about coding a secondary and refined process that will pick those designs and write some algorithms that will enable the highest-efficiency designs to be automatically reported to the user instead of manually identified. Does that make sense?
Again, thanks so much. I really, really do appreciate it.
I spent the weekend writing my speech and editing my JSHS PowerPoint presentation to my satisfaction! (For your viewing ease, my speech for each slide is in the notes in PowerPoint. There are some animations and GIFs that won't play unless you enter slide show mode, though. Either way, you'll see the whole collaboration during my presentation practice!)
I would love to hear your feedback, if possible!
Thanks so much! :)
Yes. I was so desperate for a clever blog title that I turned to Disney. ("Let It Go")
I am looking forward to practicing my presentation this coming week. I have completed and polished about 80% of my talk for the JSHS, which I will modify and condense for Gresham-Barlow.
I ordered my poster papers. I have two boards at home from years ago, so I think I will use them in constructing my poster.
Thanks for reading! More coming soon.
- Thank you for reviewing my JSHS slides with me today. I'll continue to edit up to March 9th.
- We discussed my oral presentation for all forums. I plan to write it this weekend and practice next week.
- Thank you for sending me Stephanie's comments regarding my paperwork. I would really appreciate it if you could forward me her response (if and whenever) to your response with my 2013 abstract, if only so I can rest in ease / be nervous about SRS approval beforehand. :)
IT'S SO DARN CLOSE
WORDS CANNOT EXPRESS
Right now, my paper is approaching it's penultimate form. I need to create a graph to show the differences (along with actually inserting all of my graphs into my paper), and then I can focus solely on my presentation, footnotes, and refining refining refining!!! I will go into more depth in class tomorrow (as I am dead tired because of a combination of extracirricular factors), and I can answer more fully Veronica's questions. But, anyways, here!
I have completed a first rough draft of my poster for the NWSES system. It still needs work, but I have attached the draft here for reference.
In other news, I am closing in on a first draft for my JSHS PowerPoint presentation as well.
Thank you very much!
I successfully pieced together a layout for a two-board poster, 72x48 total. The title is large and each section (introduction, methods, results, analysis, relevance, future work, conclusion, and references) is planned now. I believe the space is necessary to cover all my material, still in a concise manner but with enough detail.
Over the next weeks, I will be working on this poster and my oral presentations for all venues.
Many many thanks! :) All feedback is welcome and appreciated.
Updated in my research paper:
Afterwards, I will make the graphs and write them up in my paper.
In addition to the intro, results (figures), and conclusion components, I'm going to design a visual that will illustrate my methods (probably a flowchart).
I am starting to work on my poster for the NWSES system as well as composing my oral and PowerPoint presentation for the Oregon JSHS.
I'm going to print out two posters using PowerPoint templates from MakeSigns, paste each on a poster board, and clamp the two poster boards together for a mega-board. I've checked and it fits the NWSE size restrictions, barely. :-)
Does that sound good? Thanks so much again.
And here's my second design.
- More intuitive visual of DIFFERENCE between layers, I think - at least, that was my intention by using multi-color
- (Although my first design does depict layers with differently-sized quantum dots)
- You can't see the quantum dots
- There is no incoming sunlight (although I made the top layer yellow to invoke that)